| Other Notes | The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format. |
| | For additional information, please see www.sigma-aldrich.com/genomicdna. |
| Features and Benefits | • Typical DNA yields of 25 μg from 2 × 106 cultured cells or 30 μg from 25 mg of tissue (Fig. 4)
• Preparation time is only 20 minutes after lysis
• Purified genomic DNA has A260/A280 ratios between 1.6 and 1.9
• No need for mechanical homogenization
• 40% more purification preps offered than market leader
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| Principle | Mammalian cells and tissues are lysed with a chaotropic salt-containing buffer to ensure denaturation of macromolecules. DNA is bound to the spin column membrane and the remaining lysate is removed by centrifugation. A filtration column is used to remove cell debris, after washing to remove contaminants; the DNA is eluted with buffer into a collection tube. The purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction digestion (Fig. 3), ligation, and PCR. |
| Legal Information | GenElute is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
